Heparin, heparan sulfate
Heparinase can selectively cut the O(1-4) glycosidic bond between glucosamine and uronic acid in the sugar chain of sulfated heparin. This cleavage process is completed by elimination reaction and produces oligosaccharides containing unsaturated uronic acid residues (double bonds between C4-C5. The degradation products can be detected by an ultraviolet photometer (232nm).
EXAMPLES OFAPPLICATION
Degradation of heparin for structural analysis, such as 1,6-anhydride ring structure test of enoxaparin sodium, heparin disaccharide profile and oligosaccharide profile analysis Partially degrade heparin to obtain low molecular weight heparin, which can be chemically modified or grafted onto other materials
Degrade crude heparin to detect its source (qPCR method)
Degrade heparin and heparan sulfate to prepare heparin disaccharides and heparin oligosaccharides
Processing of plasma or other tissues for further research
Large-scale production of tineparin
Heparinase can selectively cut the O(1-4) glycosidic bond between glucosamine and uronic acid in the sugar chain of sulfated heparin. This cleavage process is completed by elimination reaction and produces oligosaccharides containing unsaturated uronic acid residues (double bonds between C4-C5. The degradation products can be detected by an ultraviolet photometer (232nm).
EXAMPLES OFAPPLICATION
Degradation of heparin for structural analysis, such as 1,6-anhydride ring structure test of enoxaparin sodium, heparin disaccharide profile and oligosaccharide profile analysis Partially degrade heparin to obtain low molecular weight heparin, which can be chemically modified or grafted onto other materials
Degrade crude heparin to detect its source (qPCR method)
Degrade heparin and heparan sulfate to prepare heparin disaccharides and heparin oligosaccharides
Processing of plasma or other tissues for further research
Large-scale production of tineparin
Main Products
heparinase, s-2238, s-2765, thrombin, UDP-GalNAz